Effects of mycorrhiza symbiosis on seed yield and some physiological responses of chickpea genotypes

Arshadi, Javad; Parsa, Mahdi; Lakzian, Amir; Kafi, Mohammad

doi:10.22034/aes.2021.144190

Effects of mycorrhiza symbiosis on seed yield and some physiological responses of chickpea genotypes

Document Type : Original research article

Authors

Javad Arshadi ¹

Mahdi Parsa ²

Amir Lakzian ³

Mohammad Kafi ²

¹ Ph.D Graduate in Crop Physiology, Department of Agronomy, Ferdowsi University of Mashhad, Mashhad, Iran

² Department of Agronomy, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

³ Department of Soil Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

10.22034/aes.2021.144190

Abstract

An experiment was conducted in 2014 in the research field of Ferdowsi University of Mashhad, Iran, to investigate the triplet symbiosis of chickpea, mycorrhiza, and rhizobium, as well as the responses of chickpea genotypes to these inoculations. The experimental design was a split-plot with three replications based on a randomized complete block design. The main plot included three mycorrhiza levels as a biological fertilizer (Glomus mosseae, Piriformospora indica, and non-used), while the subplot included nine chickpea genotypes. When compared to other treatments, G. mosseae significantly increased seed yield and dry matter of chickpeas from mid-season onward. Arbuscular mycorrhiza significantly increased chlorophyll a and b levels, as well as carotenoids and SPAD levels. MCC537 outperformed the other genotypes in terms of seed yield and dry matter during the growing season and at harvest time. MCC537, MCC427, and MCC392 genotypes had the highest levels of carotenoids and SPAD readings. It appears that using G. mosseae in conjunction with rhizobium can improve the physiological traits and seed yield of chickpea.

Highlights

In 2014, researchers in Mashhad, Iran, tested the triplet symbiosis of chickpea, mycorrhiza, and rhizobium, as well as the genotype responses.

Main plot had three mycorrhiza levels (Glomus mosseae, Piriformospora indica, and non-used), sub-plot had nine chickpea genotypes.

During the growing season and harvest, MCC537 outperformed the other genotypes in seed yield and dry matter.

Using G. mosseae with rhizobium appears to improve chickpea physiological traits and seed yield.

Keywords

Chlorophyll

Legume crops

Piriformospora indica

Rhizobium

Abbott, L.K., Murphy, D.V., 2007. Soil biological fertility: A key to sustainable land use in agriculture, Springer the Netherlands.

Andrew, J.S., Moreau, H., Kuntz, M., Pagny, G., Lin, C., Tanksley, S., McCarthy, J., 2008. An investigation of carotenoid biosynthesis in Coffea canephora and Coffea Arabica. Journal of Plant Physiology 165, 1087–1106.

Arshadi, J., Asgharipour, M.R., 2011. The Effects of Seed Size on Germination and Early Seedling Growth of Pelleted Seeds of Sugar Beet. Journal of Applied Sciences Research, 7(8), 1257-1260.

Baltruschant, H., Fodor, J., Harrach, B.D., Niemczyk, E., Barna, B., Gullner, G., Janeczko, A., Kogel, K., Schafer, P., Schwarczinger, I., 2008. Salt tolerance of barley induced by the root endophyte Piriformospora indica is associated with a strong increase in antioxidants. New Phytologist, 180, 501–510.

Martinez, D.E., Guiamet, J.J., 2004. Distortion of the SPAD 502 chlorophyll meter readings by changes in irradiance and leaf water status. Agronomie, 24, 41–46.

Diouf, D., Diop, T.A., Ndoye, I., 2003. Actinorhizal, mycorhizal and rhizobial symbioses: how much do we know?. African Journal of Biotechnology, 2(1), 1-7.

FAOSTAT. (2018). http://faostat.fao.org/faostat. Accessed 10 Sep. 2018.

Gaur, P.M., Samineni, S., Tripathi, S., Varshney, R.K. and Gowda, C.L., 2015. Allelic relationships of flowering time genes in chickpea. Euphytica, 203(2), 295-308.

Harrach, B.D., 2009. Abiotic and biotic stress effects on barley and tobacco plants, Plant Protection Institute of the Hungarian Academy of Sciences in Budapest.

IBPGR., ICRISAT., ICARDA., Description for chickpea (Cicer arietinum L.), Printed by ICRISAT, 1993.

Wu, J., Wang, D., Rosen, C.J. and Bauer, M.E., 2007. Comparison of petiole nitrate concentrations, SPAD chlorophyll readings, and QuickBird satellite imagery in detecting nitrogen status of potato canopies. Field Crops Research, 101(1), 96-103.

Kranz, C.N., McLaughlin, R.A., Johnson, A., Miller, G., Heitman, J.L., 2020. The effects of compost incorporation on soil physical properties in urban soils–A concise review. Journal of Environmental Management, 261, p.110209.

Kumar, V., Sahai, V., Bisaria, V.S., 2011. High-density spore production of Piriformospora indica, a plant growth-promoting endophyte, by optimization of nutritional and cultural parameters. Bioresource Technology, 102(3), 3169-3175.

Lucinski, R., Polcyn, W., Ratajczak, L., 2002. Nitrate reduction and nitrogen fixation in symbiotic association Rhizobium-legumes. Acta Biochimica Polonica, 49(2), 537-546.

Nikolaeva, M.K., Maevskaya, S.N., Shugaev, A.G., Bukhov, N.G., 2010. Effect of drought on chlorophyll content and antioxidant enzyme activities in leaves of three wheat cultivars varying in productivity. Russian Journal of Plant Physiology, 57(1), 87-95.

Pepó, P., Vári, E., 2016. Evaluation of the yield production of C3 (Triticum aestivum L.) and C4 (Zea mays L.) cereals with a new plant physiological parameter. Journal of Animal and Plant Sciences, 26(3), 716-724.

Sharma, A.K., 2002. Biofertilizers for sustainable agriculture. Eds., Agrobios, India.

Solaiman, A.R.M., Rabbani, M.G., Molla, M.N., 2005. Effects of inoculation of Rhizobium and arbuscular mycorrhiza, poultry litter, nitrogen, and phosphorus on growth and yield in chickpea. Korean Journal of Crop Science, 50(4), 256-261.

Stein, E., Molitor, A., Kogel, K.H., Waller, F., 2008. Systemic resistance in Arabidopsis conferred by the mycorrhizal fungus Piriformospora indica requires jasmonic acid signaling and the cytoplasmic function of NPR1. Plant and Cell Physiology, 49(11), 1747-1751.

Taiz, L., Zeiger, E., 2006. Plant Physiology. Eds., Sinauer Associates. Inc. Publishers.

Talebi, R., Ensafi, M.H., Baghebani, N., Karami, E., Mohammadi, K., 2013. Physiological responses of chickpea (Cicer arietinum) genotypes to drought stress. Environmental and Experimental Biology, 11, 9-15.

Verma, S., Varma, A., Rexer, K.H., Hassel, A., Kost, G., Sarbhoy, A., Bisen, P., Butehorn, B., Franken, P., 1998. Piriformospora indica, gen. et sp. nov., a new root-colonizing fungus. Mycologia, 90(5), 896-903.

Voisin, A.S., Gueguen, J., Huyghe, C., Jeuffroy, M.H., Magrini, M.B., Meynard, J.M., Mougel, C., Pellerin, S., Pelzer, E., 2014. Legumes for feed, food, biomaterials and bioenergy in Europe: a review. Agronomy for Sustainable Development, 34(2), 361-380.

Wellbum, A.R., 1994. The spectral determination of chlorophyll a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Journal of Plant Physiology, 144(3), 307–313.

Zaidi, A., Khan, M.S. and Amil, M.D., 2003. Interactive effect of rhizotrophic microorganisms on yield and nutrient uptake of chickpea (Cicer arietinum L.). European Journal of Agronomy, 19(1), 15-21.