This study aimed to investigate the dynamics of flowering and evaluate the effect of open and self-pollination on the fertility of seedless barberry commercial clone shrubs in Iran. During the bud swelling stage, ten shrubs were selected, and on each shrub, one branch bearing at least 100 flower buds was chosen to monitor flowering dynamics through daily recordings of bud and open flower counts. To assess fertility, two branches bearing at least 100 flower buds were chosen on each shrub. One branch was isolated with cotton fabric bags for self-pollination treatment, while the other remained unisolated for open-pollination, with the initial and final fruit set (IFF, FFF) subsequently recorded for both. Concurrently, pollen viability was evaluated by measuring pollen germination. The flowering period ranged from 16-19 days. Under open-pollination conditions, the IFF was 70-80% and FFF was 50-60%. Under controlled self-pollination conditions in bagged shoots, IFF was 50-60% and FFF was 40-50%. The high fruit set in isolated branches suggests the capability for parthenocarpic fruit development stimulated by self-pollination. However, the significantly higher fruit set in open-pollination underscores the critical role of pollinator insects in enhancing pollination efficiency. Fruit drop was 20.11% and 13.37% for open- and self-pollinated treatments, respectively. This research had no specific limitations. This research provides practical and valuable information into pollination and fertility in this seedless barberry, supporting the adoption of optimal orchard management practices to enhance fertility outcomes for future applications.